The purpose of this study is to evaluate the thermo-physiological comfort of weft knitted fabrics and to investigate applied methods of heat and water transfer. The examined weft knitted fabrics had a different structure as well as a material composition. They were made for wearing in moderate alternatively hot climate conditions such as in Europe. Selected properties were measured on the C-Therm Tci and Moisture Management Tester (MMT) devices. We concluded that the weft knitted fabrics made of polyester, of greater thickness and specific density are comfortable in hot and moderate climate conditions. The most suitable alternative is to combine structures and materials in the manufacturing process of knitted products.