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This article presents an analysis of the internal damping changes depending on the temperature of the austenitic stainless steels. The measurement of internal damping has a great meaning in investigation and evaluation of microplasticity and fatigue processes in metals. In experimental measurements only resonance method was used, which is based on continuous excitation of oscillations of the specimen and the entire apparatus vibrates at a frequency which is near to the resonance. These mechanisms have been studied by ultrasonic resonant apparatus. During the internal damping measurement of austenitic stainless steel it was found that the steels had characterized peak around the temperature of 100 °C and the backward measurement, by cooling down the sample, does not create any peak.